Abstract:

In order to study the effects of antioxidants on growth and metabolism under oxidative stress, different types and doses of antioxidants were added in the fermentation medium of Zymomonas mobilis strain ATCC31821. It was found that pyrocatechol was more useful for growth and ethanol fermentation of Z. mobilis, and improving anti-oxidation stress ability. The optimized conditions obtained by orthogonal test were as follows, pH 5, 200 ml liquid volume, 100 r/min shaker frequency and 0.1 mg/ml pyrocatechol concentration. The maximum ethanol yield could reach 40.16 g/L under the optimized condition.

Key words: Zymomonas mobilis, oxidative stress, antioxidant, fermentation, ethanol